Deshpande Lab 

 

Khateb, A., Deshpande, A., Feng, Y., Lee, J. S., Lazar, I., Fabre, B., Li, Y., Finlay, D., Fujita, Yu, Z., Tongwu, Y., Jun, Pass, I., Livneh, I., Burian, C., Mason, J. R., Almog, R., Horesh, N., Ofran, Y., Brown, K., Vuori, K., Jackson, M., Ruppin, E., Deshpande, A. J., and Ronai, Z. A.  RNF5 Regulation of RBBP4 Defines Acute Myeloid Leukemia Growth and Susceptibility to Histone Deacetylase Inhibitors Nat Commun. 2021 (in press)

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Klein B.J.*, Deshpande A.*, Khan C.L., Xuan F., Zandian M., Barbosa K., Khanal S., Tong Q., Zhang Y., Zhang P., Sinha A., Bohlander S.K., Shi X., Wen H., Poirier M.G., Deshpande AJ. And Kutateladze T.G. The role of the PZP domain of AF10 in acute leukemia driven by AF10 translocations. Nat Commun. 2021 Jul5;12(1):4130. Doi: 10.1038/s41467-021-24418-9. *equal contribution

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Chen B-R, Deshpande A, Barbosa K, Kleppe M, Li X, Yeddula N, Campos AR, Wechsler-Reya RJ, Bagchi A, Meshinchi S, Eaves C, Jeremias I, Haferlach T, Frank DA, Ronai Ze, Chanda S, Armstrong SA, Adams PD, Levine RL, Deshpande AJ. A JAK/STAT-Mediated Inflammatory Signaling Cascade Drives Oncogenesis In AF10-Rearranged AML. Blood, (in press).

 

Kingsley MC, Xie HM, Chen BR, Riedel SS, Pastuer T, Bollig MK, Shank T, Libbrecht C, Stabler SP, Deshpande AJ, Intlekofer AM, Bernt KM. Specific patterns of H3K79 methylation influence genetic interaction of oncogenes in AML. Blood Adv. 2020;4(13):3109-22. Epub 2020/07/08. doi: 10.1182/bloodadvances.2020001922. PubMed PMID: 32634241; PMCID: PMC7362367.

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Tiwari A, Tashiro K, Dixit A, Soni A, Vogel K, Hall B, Shafqat I, Slaughter J, Param N, Le A, Saunders E, Paithane U, Garcia G, Campos AR, Zettervall J, Carlson M, Starr TK, Marahrens Y, Deshpande AJ, Commisso C, Provenzano PP, Bagchi A. Loss of HIF1A From Pancreatic Cancer Cells Increases Expression of PPP1R1B and Degradation of p53 to Promote Invasion and Metastasis. Gastroenterology. 2020;159(5):1882-97 e5. Epub 2020/08/10. doi: 10.1053/j.gastro.2020.07.046. PubMed PMID: 32768595; PMCID: PMC7680408.

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Garancher, A., Suzuki H., Haricharan, S., Chau, L.Q., Masihi, MB Rusert, J.M.,  Norris, P.S., Carrette, F.,  Romero, M.M., Morrissy, S.A., Skowron, P., Cavalli, F.M.G, Farooq2, H., Ramaswamy,V.,  Jones, S.J.M., Moore R.A., Mungall, A.J., Ma, Y., Thiessen, N.,  Li, Y., Morcavallo, A.,  Qi, L., Kogiso, M., Du Y., Baxter, P.,  Henderson, J.J., Crawford, J.R., Levy, M.L., Olson, J.M., Cho, Y-J., Deshpande, A.J., Li, X-N., Chesler, L., Marra, M.A., Wajant, H., Becher, O.J., Bradley, L.M., Ware, C.F., Taylor, M.D. and Wechsler-Reya, R.J. Tumor Necrosis Factor Overcomes Immune Evasion in p53-Mutant Medulloblastoma Nature Neuroscience 2020 (in press)

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Barbosa K, Li S, Adams PD and Deshpande AJ. The Role of TP53 in Acute Myeloid Leukemia: Challenges and Opportunities. Gene Chromosome Canc. 2019 Aug 8. doi: 10.1002/gcc.22796.

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Lytle NK, Ferguson LP, Rajbhandari N1, Gilroy K, Fox RG, Deshpande A, Schürch CM, Hamilton M, Robertson N, Lin W, Noel P, Wartenberg M, Zlobec I, Eichmann M, Galván JA, Karamitopoulou E, Gilderman T, Esparza LA, Shima Y, Spahn P, French R, Lewis NE, Fisch KM, Sasik R, Rosenthal SB, Kritzik M, Von Hoff D, Han H, Ideker T, Deshpande AJ, Lowy AM, Adams PD, Reya T.  A Multiscale Map of the Stem Cell State in Pancreatic Adenocarcinoma. Cell 2019 Apr 18;177(3):572-586.e22. doi: 10.1016/j.cell.2019.03.010.

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Barbosa K, Deshpande A, Chen BR, Ghosh A, Sun Y, Dutta S, Weetall M, Dixon J, Armstrong SA, Bohlander SK, Deshpande AJ. Acute Myeloid Leukemia Driven by the CALM-AF10 Fusion Gene is Dependent on BMI1 Exp Hematol. 2019 Jun;74:42-51.e3. doi: 10.1016/j.exphem.2019.04.003

 

Deshpande, A., Chen, B. R., Zhao, L., Saddoris, K., Kerr, M., Zhu, N., Mali, P., Deshpande, A. J. Investigation of Genetic Dependencies Using CRISPR-Cas9-based Competition Assays. J. Vis. Exp. (143), e58710, doi:10.3791/58710 (2019).

 

Sun Y, Chen BR, Deshpande A.J. Epigenetic Regulators in the Development, Maintenance, and Therapeutic Targeting of Acute Myeloid Leukemia. Front Oncol. 2018;8:41. doi: 10.3389/fonc.2018.00041. PubMed PMID: 29527516; PMCID: PMC5829038.

 

Numata A, Kwok HS, Kawasaki A, Li J, Zhou QL, Kerry J, Benoukraf T, Bararia D, Li F, Ballabio E, Tapia M, Deshpande AJ, Welner RS, Delwel R, Yang H, Milne TA, Taneja R, Tenen DG. The basic helix-loop-helix transcription factor SHARP1 is an oncogenic driver in MLL-AF6 acute myelogenous leukemia. Nat Commun. 2018;9(1):1622. doi: 10.1038/s41467-018-03854-0. PubMed PMID: 29692408; PMCID: PMC5915391.

 

Cusan M, Vegi NM, Mulaw MA, Bamezai S, Kaiser LM, Deshpande AJ, Greif PA, Quintanilla-Fend L, Gollner S, Muller-Tidow C, Humphries KR, Armstrong SA, Hiddemann W, Feuring-Buske M, Buske C. Controlled stem cell amplification by HOXB4 depends on its unique proline-rich region near the N terminus. Blood. 2017;129(3):319-23. doi: 10.1182/blood-2016-04-706978. PubMed PMID: 27827825.

 

Valerio DG, Xu H, Chen CW, Hoshii T, Eisold ME, Delaney C, Cusan M, Deshpande AJ, Huang CH, Lujambio A, Zheng YG, Zuber J, Pandita TK, Lowe SW, Armstrong SA. Histone acetyltransferase activity of MOF is required for MLL-AF9 leukemogenesis. Cancer Research. 2017. doi: 10.1158/0008-5472.CAN-16-2374. PubMed PMID: 28202522.

 

Kuhn, M. W., Song, E., Feng, Z., Sinha, A., Chen, C. W., Deshpande, A. J., Cusan, M., Farnoud, N., Mupo, A., Grove, C., et al. (2016). Targeting Chromatin Regulators Inhibits Leukemogenic Gene Expression in NPM1 Mutant Leukemia. Cancer Discov.

 

Zhu, N., Chen, M., Eng, R., DeJong, J., Sinha, A. U., Rahnamay, N. F., Koche, R., Al-Shahrour, F., Minehart, J. C., Chen, C.-W., Deshpande, A. J., Xu, H., Chu, S. H., Ebert, B. L., Roeder, R. G., Armstrong, S. A. MLL-AF9- and HOXA9-mediated acute myeloid leukemia stem cell self-renewal requires JMJD1C. J Clin Invest 2016.

 

Arreba-Tutusaus P, Mack T., Bullinger L., Schnöder T., Polanetzki A., Weinert S., Ballaschk A., Wang Z., Deshpande A.J., Armstrong S.A., Döhner K., Fischer T., and Heidel F. Impact of FLT3-ITD location on sensitivity to TKI-therapy in vitro and in vivo Leukemia (in press)

 

Chen, S., Yang, Z., Wilkinson, Alex W., Deshpande, A. J., Sidoli, S., Krajewski, K., Strahl, Brian D., Garcia, Benjamin A., Armstrong, Scott A., Patel, Dinshaw J., and Gozani, O. The PZP Domain of AF10 Senses Unmodified H3K27 to Regulate DOT1L-Mediated Methylation of H3K79. Mol Cell 2015, 60(2):319-27.

 

Chen C.W., Koche R.P., Sinha A.U., Deshpande A.J., Zhu N., Eng R., Doench, J.G., Xu H., Chu S.H., Qi J., Bernt, K.M., Root, D.E., Hahn, W.C., Bradner J.E., Armstrong S.A. 2015. DOT1L inhibits SIRT1-mediated epigenetic silencing to maintain leukemic gene expression in MLL-rearranged leukemia. Nature Medicine, 21, 335-343.

 

Deshpande, A.J., Deshpande A., Sinha A.U., Chen L., Chang J., Chen D., Zhu N., Dzhekieva, L., Ibáñez G, Dias S., Banka D, Krivtsov A., Luo, M., Bradner J.E., Bernt K.B., and Armstrong S.A. 2014. AF10 Regulates Progressive H3K79 Methylation and HOX Gene Expression in Diverse AML Subtypes, Cancer Cell, 26, 896-908

 

Santos, M.A., Faryabi, R.B., Ergen, A.V., Day, A.M., Malhowski, A., Canela, A., Onozawa, M., Lee, J.-E., Callen, E., Gutierrez-Martinez, P., Chen, H.-T., Wong, N., Finkel, N., Deshpande, A.J, Sharrow, S., Rossi, D.J., Ito, K., Ge, K., Aplan, P.D., Armstrong, S.A., Nussenzweig, A., 2014. DNA-damage-induced differentiation of leukaemic cells as an anti-cancer barrier. Nature Jul 27. Doi: 10.1038/nature13483 [Epub ahead of print] PMID Journal – In Process.

 

Deshpande A.J., Bradner J.E. and Armstrong S.A. (2012) Chromatin modifications as therapeutic targets in MLL-rearranged leukemia, Trends in Immunol.33, 563-570.

 

Deshpande, A. J. *, Chen, L*, Fazio, M., Sinha, A.U., Bernt, K.M., Banka, D., Dias, S., Chang, J., Olhava, E.J., Daigle, S.R., Richon, V.M., Pollock, R.M., Armstrong, S.A., 2013. Leukemic transformation by the MLL-AF6 fusion oncogene requires the H3K79 methyltransferase Dot1l. Blood 121, 2533-2541.

 

Chen, L., Deshpande, A. J., Chen, L., Banka, D., Bernt, K.M., Dias, S., Buske, C., Olhava, E.J., Daigle, S.R., Richon, V.M., Pollock, R.M., Armstrong, S.A., (2013). Abrogation of MLL-AF10 and CALM-AF10-mediated transformation through genetic inactivation or pharmacological inhibition of the H3K79 methyltransferase Dot1l. Leukemia 27, 813-822.

 

Deshpande, A.J.*, Rouhi, A. *, Lin, Y., Stadler, C., Greif, P.A., Arseni, N., Opatz, S., Quintanilla-Fend, L., Holzmann, K., Hiddemann, W., Dohner, K., Dohner, H., Xu, G., Armstrong, S.A., Bohlander, S.K., Buske, C., 2011. The clathrin-binding domain of CALM and the OM-LZ domain of AF10 are sufficient to induce acute myeloid leukemia in mice. Leukemia 25, 1718-1727.

 

Mulaw, M.A., Krause, A., Deshpande, A.J., Krause, L.F., Rouhi, A., La Starza, R., Borkhardt, A., Buske, C., Mecucci, C., Ludwig, W.D., Lottaz, C., Bohlander, S.K., 2012. CALM/AF10-positive leukemias show upregulation of genes involved in chromatin assembly and DNA repair processes and of genes adjacent to the breakpoint at 10p12. Leukemia 26, 1012-1019.

 

Deshpande, A., Pastore, A., Deshpande, A. J., Zimmermann, Y., Hutter, G., Weinkauf, M., Buske, C., Hiddemann, W., and Dreyling, M. (2009). 3'UTR mediated regulation of the cyclin D1 proto-oncogene. Cell Cycle 8, 3584-3592.

 

Deshpande, A.J., Ahmed, F.A., and Buske, C. (2009) Identification of Murine and Human Acute Myeloid Leukemia Stem Cells. In: Yu, J.S., ed. Cancer Stem Cell Methods, Humana Press.

 

Lin, Y.H., Kakadia, P.M., Chen, Y., Li, Y.Q., Deshpande, A.J., Buske, C., Zhang, K.L., Zhang, Y., Xu, G.L., Bohlander, S.K., 2009. Global reduction of the epigenetic H3K79 methylation mark and increased chromosomal instability in CALM-AF10-positive leukemias. Blood 114, 651-658.

 

Archangelo, L.F., Greif, P.A., Holzel, M., Harasim, T., Kremmer, E., Przemeck, G.K., Eick, D., Deshpande, A.J., Buske, C., de Angelis, M.H., Saad, S.T., Bohlander, S.K., 2008. The CALM and CALM/AF10 interactor CATS is a marker for proliferation. Mol Oncol 2, 356-367.

 

Deshpande, A.J. and Buske, C. Cancer stem cells in hematopoietic malignancies In: Zaenker K.S. and Dittmar, T., ed. Stem Cells and Cancer Nova Press.

 

Petropoulos, K., Arseni, N., Schessl, C., Stadler, C.R., Rawat, V.P., Deshpande, A.J., Heilmeier, B., Hiddemann, W., Quintanilla-Martinez, L., Bohlander, S.K., Feuring-Buske, M., Buske, C., 2008. A novel role for Lef-1, a central transcription mediator of Wnt signaling, in leukemogenesis. J Exp Med 205, 515-522.

 

Deshpande, A.J. and Buske, C. Molecular Oncogenesis of Leukemia In: Dunphy, C.H., ed. Molecular Pathology of Hematolymphoid Diseases Springer Verlag.

 

Rawat, V.P., Thoene, S., Naidu, V.M., Arseni, N., Heilmeier, B., Metzeler, K., Petropoulos, K., Deshpande, A., Quintanilla-Martinez, L., Bohlander, S.K., Spiekermann, K., Hiddemann, W., Feuring-Buske, M., Buske, C., 2008. Overexpression of CDX2 perturbs HOX gene expression in murine progenitors depending on its N-terminal domain and is closely correlated with deregulated HOX gene expression in human acute myeloid leukemia. Blood 111, 309-319.

 

Deshpande, A. J., and Buske, C. (2007). Knocking the Wnt Out of the Sails of Leukemia Stem Cell Development

Cell Stem Cell, doi:10.1016/j.stem.2007.11.006.

 

Deshpande, A. J., and Buske, C. (2007). Lymphoid progenitors as candidate cancer stem cells in AML: new perspectives. Cell Cycle 6, 543-545.

 

Deshpande, A. J., Cusan, M., Rawat, V.P., Reuter, H., Krause, A., Pott, C., Quintanilla-Martinez, L., Kakadia, P., Kuchenbauer, F., Ahmed, F., Delabesse, E., Hahn, M., Lichter, P., Kneba, M., Hiddemann, W., Macintyre, E., Mecucci, C., Ludwig, W.D., Humphries, R.K., Bohlander, S.K., Feuring-Buske, M., Buske, C., 2006. Acute myeloid leukemia is propagated by a leukemic stem cell with lymphoid characteristics in a mouse model of CALM/AF10-positive leukemia. Cancer Cell (featured article) 10, 363-374.

 

Schessl, C., Rawat, V.P., Cusan, M., Deshpande, A., Kohl, T.M., Rosten, P.M., Spiekermann, K., Humphries, R.K., Schnittger, S., Kern, W., Hiddemann, W., Quintanilla-Martinez, L., Bohlander, S.K., Feuring-Buske, M., Buske, C.,. The AML1-ETO fusion gene and the FLT3 length mutation collaborate in inducing acute leukemia in mice. J Clin Invest 115, 2159-2168

 

Rawat, V. P., Cusan, M., Deshpande, A. J., Hiddemann, W., Quintanilla-Martinez, L., Humphries, R. K., Bohlander, S. K., Feuring-Buske, M., and Buske, C. (2004). Ectopic expression of the homeobox gene Cdx2 is the transforming event in a mouse model of t(12;13)(p13;q12) acute myeloid leukemia. Proc Natl Acad Sci U S A 101, 817-822.